Bacteria are well-known for their ability to adapt to any given environment. Scientists had no idea that bacteria could employ a type of sponge to soak up signals. The research was just published in the ‘Molecular Cell journal.
IMIB and Helmholtz RNA-based Infection Research Institute were both involved in the study. Jorg Vogel, a professor of molecular biology at JMU and HIRI director, discovered new insights about these signaling routes and processes in his laboratory. Antibiotic-resistant bacteria kill at least 1.28 million people per year, according to research published in The Lancet. The scientists predicted that by 2050, this figure may climb to as many as 10 million.
This heightened the urgency of the search for novel chemicals that can combat resistant bacterial strains. RNA-based antibiotics with programmability are one possibility. In-depth knowledge of the essential RNA-based signaling pathways and processes during infection was necessary for this to be accomplished. Jorg Vogel’s co-author Gianluca Matera, IMIB’s Ph.D. student, offered further details on the paper’s origins. He stated Escherichia coli and Salmonella enterica are two examples of bacteria that have a cell envelope made of two membranes. The primary role of this envelope is to protect the bacteria from their environment, but it has to be permeable to nutrients that the bacteria require to grow.
Multiple RNA entities interact to control which compounds enter through the cell membrane and which are prevented, allowing bacteria to resist drugs. In Salmonella enterica, the researchers discovered an “RNA sponge,” unknown. “Small RNAs” include sponges like these. Based on this research, it appears that OppX, an RNA sponge, mimics the binding target in the outer membrane of bacteria, called MicF sRNA. It is then intercepted by OppX. Alternatively, if it were a sponge, it quickly took it in.
The sRNA MicF performed a vital role for the bacterial envelope to function. For the bacterial envelope to function, both the outside and interior membranes must work. As a result, systems for facilitating communication between them are required. One family of these regulators is the small non-coding RNA (MicF). As Gianluca Matera pointed out, As a result of using a novel method created at the Hebrew University of Jerusalem, a young scientist was able to identify Salmonella sRNAs’ interactions with each other in onRNAS
According to Matera, ” OppX improves membrane permeability by raising the expression of one of the major pores in the bacterial outer membrane,” this intercepting process has the following impact on the bacterial membrane: OmpF is the official scientific name for this pore. A bacterium’s development will be hampered if it does not have the OppX sponge, which is true in environments with few nutrients. If adequate levels of OppX are present, the membrane’s OmpF pores become more active, enhancing the intake of nutrients if they are scarce.
The OmpF pores assume a vital function when the bacteria are targeted by antibiotics. The chemicals utilize them as their principal ports of entry into the cell. “OppX might influence antibiotic efficacy by increasing OmpF synthesis and the absorption of the antibiotic itself,” Matera said. A new study suggests that OppX is a sponge for the MicF sRNA, making it the first identified regulator of MicF’s activity. According to the study, this information is crucial to comprehend MicF’s cellular action.
Based on bacteria investigations cultivated in vitro under laboratory settings, these discoveries have emerged. The next step will be to carry out similar experiments under more “realistic” circumstances. The first step has been taken in this direction: “We are decoding the Salmonella RNA interactome in infected host cells,” Jorg Vogel said. Our fundamental study aims to contribute to the creation of novel treatments because of antibiotic resistance, which is a serious health problem in our day. (ANI)